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陳文逸 教授 Wen-Yi Chen
從聘系所: 化材系主聘
專線電話:03-4227151轉34222
傳真電話:
E-mail :wychen@ncu.edu.tw
學經歷

學歷:

美國奧克拉荷馬州立大學化學工程博士


論文著作區
Journal

1. Kuo TC and Chen WY “The use of a kinesin-cro fusion protein as the nano shuttle to transport specific DNA” Current Nanoscience (in press, accepted 2012/05/10)

2. Lin SF, Chen WY, Chang TY “Sensitive metal layer assisted guided mode resonance biosensor with a spectrum inversed response and strong asymmetric resonance field distribution” Optics Express, 2012 (Accepted) (SCI)

3. Tsai, CW, Chen WY, Ruaan RC “Retention Prediction of Peptide Enantiomers in Reversed-Phase Liquid Chromatography Assisted by Molecular Dynamics Simulation” Langmuir, 2012 (Submitted) (SCI)

4. Chen WY*, Lin SF, Chang, CY, “光學免標定式波導共振生物感測器與其應用”化工技術月刊, 2012 (六月刊)

5. Lin PH, Tsai CW, Wu J.W., Ruaan RC, and Chen WY*, “Molecular Dynamics Simulation of Induced-Fit Binding Process of DNA Aptamer and L-Argininamide”, Journal of Biotechnology”, 2012 (Accepted) (SCI)

6. Tsai CW, Kao WH, Chang LC, Ruaan RC, and Chen WY*, “Structural Stability-Chromatographic Retention Relationship on Exenatide Diastereomers Separation”, Analytical and Bioanalytical Chemistry 2012 (revised) (SCI)

7. Tsao CW, Lin CH, Cheng YC, Chien CC, Chang CP and Chen WY* “Nanostructured silicon surface modifications for as a selective matrix-free laser desorption/ionization mass spectrometry” Analysts, 2012 (Accepted DOI: 10.1039/c2an35189g) (SCI)

8. Kuo TC, Lee PC and Chen WY* “The nonclassical aptamer-binding thermodynamics and stoichiometry and the accompanied structural changes of streptavidin protein” Journal of Physical Chemistry B, 2012 (Submitted) (SCI)

9. Lin SF, Wang CM, Tsai YL, Ding TJ, Yang TH, Chen WY, and Chang JY, “A model for fast predicting and optimizing the sensitivity of surface-relief guided mode resonance sensors”, Sensors and Actuator B. 2012(Accepted) (SCI)

10. Chiang YC, Chang Y, Chen WY, and Ruaan RC, “Biofouling Resistance of Ultrafiltration Membranes Controlled by Surface Self-Assembled Coating with PEGylated Copolymers”, Langmuir 28, 1399-1407 (2012) (SCI)

11. Hu WP, Huang LY, Kuo TC, Hu WW, Chang Y, Chen CS, Chen HC, Chen WY* “Optimization of DNA Directed Immobilization on Mixed oligo (ethylene glycol) (OEG) Monolayers for Immunodetection”, Analytical Biochemistry 423, 26–35 (2012) (SCI)

12. Cheng YC, Chen KH, Wang JS, Hsu WL, Chien CC, Chen WY, and Tsao CW, “Rapid Analysis of Abused Drugs Using Nanostructured Silicon Surface Assisted Laser Desorption/Ionization Mass Spectrometry”, Analyst 137 (3)654-661 (2012) (SCI)

13. Chen WY, Huang JT, Cheng YC, Chien CC, and Tsao CW, “Fabrication of nanostructured silicon by metal-assistend etching and its effects on matrix-free laser desorption/ionization mass spectrometry”, Analytica Chimica Acta, 687, 97-104, (2011) (SCI)

14. Chen WY*, Huang LY, and Hu WP, “表面電漿共振生物感測器簡件與運用”,化工, 58 (4),3-17 (2011)

15. Wang SSS, Lin MS, Fang YH, Lee KW, Chen SL, and Chen WY*, “Using isothermal titration calorimetry to real-time monitor the heat of metabolism: a case study using PC12 cells and Aβ (1-40)”, Colloid and Surface B: Biointerface, 83, 307-312, (2011) (SCI)

16. Liu JT, Lin PS, Hsin YM, Tsai JZ, Chen WY “Surface plasmon resonance biosensor for microalbumin detection” Journal of the Taiwan Institute of Chemical Engineers, 42, 696-700 (2011) (SCI)

17. Lin SF, Ding TJ, Liu JT, Lee CC, Yang TH, Chen WY* and Chang JY* “A Guided Mode Resonance Aptasensor for Thrombin Detection” Sensors 11, 8953-8965 (2011) (SCI)

18. Lue JH, Ding TJ, Yang TH, Chang JY, and Chen WY “Real-time Monitoring on Peptide Synthesis by GMR Biosensors” Proceedings - 2011 4th International Conference on Biomedical Engineering and Informatics, BMEI 4, 1858-1861 (2011) (SCI)

19. Tsai, J.-Z., Chen, C.-J., Liu, J.-T., Hsin, Y.-M., Chen, WY, Hsueh, K.-H. “Development of capacitance measurement system for human serum albumin detection” Electronics Letters 46 (10) , 678-680 (2010)

20. Huang HI, Cheng YC, Chen WY, Tsao CW, Huang CJ, Liu HT, and Chien CC*, “Comparative proteomic analysis of Placenta-Derived Multipotent Cells (PDMCs) and its differentiated Neuron”, The Chinese Journal of Pain, 20, 1-11, (2010) (SCI)

21. Lin PH, Kao YH, Chang Y, Cheng YC, Chien CC, and Chen WY*, “Daunomycin interaction with DNA: Microcalorimetric studies of the thermodynamics and binding mechanism”, Biotechnology Journal, 5, 1069-1077, (2010) (SCI)

22. Chang Y*, Yandi W, Chen WY, Shih YJ, Yang CS, Chang Y, Ling QD, and Higuchi A, “Tunable Bioadhesive copolymer hydrogels of thermoresponsive poly(N-isopropyl acrylamide) containing zwitterionic polysulfobetaine”, Biomacromolecules, 11, 1101-1110, (2010) (SCI)

23. Hung YT, Lin MS, Chen WY, and Wang SSS*, “Investigating the Effects of Sodium Dodecyl Sulfate on the Aggregative Behavior of Hen Egg-White Lysozyme at Acidic pH”, Colloids and Surfaces B: Biointerfaces, 81, 141-151, (2010) (SCI)

24. Higuchi A, Siao YD, Yang ST, Hsieh PV, Fukushima H, Chang Y, and Chen WY*, “Peroxidase Activity of DNA Aptamer-Pt Complexes Prepared with Cisplatin”, Journal of Biomaterials Science: Polymer Edition, 21, 67-82, (2010) (SCI)

25. Chang Y, Shu SH, Shih YJ, Chu CW, Ruaan RC, and Chen WY*, “Hemocompatible Mixed-Charge Copolymer Brushes of Pseudozwitterionic Surfaces Resistant to Nonspecific Plasma Protein Fouling”, Langmuir, 26, 3522–3530, (2010) (SCI)

26. Roselin LS, Lin MS, Lin PH, Chang Y, and Chen WY*, “Recent trends and some applications of Isothermal Titration Calorimetry in Biotechnology”, Biotechnology Journal, 5, 85-98, (2010) (SCI)

27. Chang Y*, Chu WL, Chen WY, Zheng J, Liu L, Ruaan RC, and Higuchi A, “A systematic SPR study of human plasma protein adsorption behavior on the controlled surface packing of self-assembled poly(ethylene oxide) triblock copolymer surfaces”, Journal of Biomedical Materials Research: Part A, 93, 400-408, (2010) (SCI)

28. Higuchi A*, Yang ST, Li PT, Ruaan RC, Chen WY, Chang Y, Chang Y, Tasi EM, Chen YH, Wang HC, Hsu ST, and Ling QD, “Permeation of blood cells from umbilical cord blood through surface-modified polyurethane foaming membranes”, Journal of Membrane Science, 339, 184-188, (2009) (SCI)

29. Chang Y*, Chen WY, Yandi W, Shih YJ, Chu WL, Liu YL, Ruaan RC, and Higuchi A, “Dual-Thermoresponsive Phase Behavior of Blood Compatible Zwitterionic Copolymers Containing Nonionic Poly(N-isopropyl acrylamide)”, Biomacromolecules, 10, 2092-2100, (2009) (SCI)

30. Chiang YC, Chang Y*, Higuchi A, Chen WY, and Ruaan RC*, “Sulfobetaine-grafted poly(vinylidene fluoride) ultrafiltration membranes exhibit excellent antifouling property”, Journal of Membrane Science, 339, 151-159, (2009) (SCI)

31. Higuchi A*, Yang ST, Li PT, Chen H, Ruaan RC, Chen WY, Chang Y, Chang Y, Tsai EM, Wang HC, and Shih-Tien Hsu, “Separation of Hematopoietic Stem and Progenitor Cells from Human Peripheral Blood Through Polyurethane Foaming Membranes Modified with Several Amino Acids”, Journal of Applied Polymer Science, 144, 671-679, (2009) (SCI)

32. Lin PH, Tong SJ, Louis SR, Chang Y, and Chen WY*, “Thermodynamic basis of chiral recognition in a DNA aptamer”, Physical Chemistry Chemical Physics, 11, 9744-9750, (2009) (SCI)

33. Tasi JZ, Chen CJ, Chen WY, Liu JT, Liao CY, and Hsin YM*, “A new PZT piezoelectric sensor for gravimetric applications using the resonance-frequency detection”, Sensors & Actuators B: Chemical, 139, 259-264, (2009) (SCI)

34. Lin MS, Chen XB, Wang SSS, Higuchi A, Chang Y, and Chen WY*, “Dynamic fluorescence imaging analysis to investigate the cholesterol recruitment in lipid monolayer during the interaction between beta-amyloid (1-40) and lipid monolayers”, Colloid and Surface B, 74, 59-66, (2009) (SCI)

35. Higuchi A, Yang ST, Siao YD, Hsieh PV, Fukushina H, Chang Y, and Chen WY*, “Preparation of fractioned DNA aptamer–Pt complex through ultrafiltration and the colorimetric sensing of thrombin”, Journal of Membrane Science, 328, 97-103, (2009) (SCI)

36. Higuchi A, Siao YD, Yang ST, Hsieh PV, Fukushima H, Chang Y, Ruaan RC, and Chen WY*, “Preparation of a DNA aptamer-Pt complex and its use in the colorimetric sensing of thrombin and anti-thrombin antibodies”, Analytical Chemistry, 80, 6580-6586, (2008) (SCI)

37. Lin PH, Yen SL, Lin MS, Chang Y, Louis SR, Higuchi Akon, and Chen WY*, “Microcalorimetrics Studies of the Thermodynamics and Binding Mechanism between l-Tyrosinamide and Aptamer”, Journal of Physical and Chemistry B, 112, 6665-6673, (2008) (SCI)

38. Lin MS, Tsai HT, Wang SSS, Chang Y, and Chen WY*, “Investigation of the mechanism of beta-amyloid fibril formation by kinetic and thermodynamic analyses”, Langmuir, 24, 5802-5808, (2008) (SCI)

39. Chang Y*, Shih YJ, Ruaan RC, Higuchi A, Chen WY, and Lai JY, “Preparation of poly(vinylidene fluoride) microfiltration membrane with uniform surface-copolymerized poly(ethylene glycol) methacrylate and improvement of blood compatibility”, Journal of Membrane Science, 309, 165-174, 2008, (SCI)

40. Higuchi A*, Chen WY, Yamamoto T, Gomei Y, Fukushima H, Chang Y, and Ruaan RC, “Preservation of Hematopoietic Stem and Progenitor Cells from Umbilical Cord Blood Stored in a Surface Derivatized with Polymer Nanosegments”, Biomacromolecules, 9, 634-639, (2008) (SCI)

41. Liu JT, Chen LY, Shih MC, Chang Y, and Chen WY*, “The investigation of recognition interaction between phenylboronate monolayer and glycated hemoglobin using surface plasmon resonance”, Analytical Biochemistry, 375, 90-96, (2008) (SCI)

42. Chang Y, Liao SC, Higuchi A, Ruaan RC, Chu CW, and Chen WY*, “A Highly Stable Nonbiofouling Surface with Well-Packed Grafted Zwitterionic Polysulfobetaine for Plasma Protein Repulsion”, Langmuir, 24, 5453-5458, (2008) (SCI)

43. Lin MS, Tsai HT, Wang SSS, Chang Y, and Chen WY*, “Examining the levels of ganglioside and cholesterol in cell membrane on attenuation the cytotoxicity of beta-amyloid peptide”, Colloids and Surfaces B: Biointerfaces, 65, 172-177, (2008) (SCI)

44. Higuchi A*, Sekiya M, Gomei Y, Sakurai M, Chen WY, Egashira S, and Matsuoka Y, “Separation of hematopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes”, Journal of Biomedical Materials Research: Part A, 85, 853-861, (2008) (SCI)

45. Chen WY*, Liu ZC, Lin PH, Fang CI, and Yamamoto S, “The Hydrophobic Interactions of the Ion-exchanger Resin Ligands with Proteins at High Salt Concentrations by Adsorption Isotherms and Isothermal Titration Calorimetry”, Separation and Purification Technology, 54, 212-219, (2007) (SCI)

46. Chen WY*, Lin MS, Tasi PS, Liu JT , Lin PH, and Yamamoto S, “Studies of the Interaction Mechanism between single strand and double strand DNA with Hydroxyapatite by Microcalorimetry and Isotherm Measurements”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 295, 274-283, (2007) (SCI)

47. Chen LY, Lin JJ, Chiu SM, and Chen WY*, “Kinetics and Morphology Analysis of the Effects of Lipid Composition on Fabrication a Mimetic Bio-Membrane on Brij-76 Derived Surface”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 296, 86-91, (2007) (SCI)

48. Chen WY, Hu WP, Su YD, Taylor A, Jiang S, and Chang GL*, “A multispot DNA chip fabricated with mixed ssDNA/Oligo (ethylene glycol) self-assembled monolayers for detecting the effect of secondary structures on hybridization by SPR imaging”, Sensor and Actuator B: Chemical, 125, 607-614, (2007) (SCI)

49. Lin MS, Chiu HM, Fan FJ, Tsai HT, Wang SSS, Chang Y, and Chen WY*, “Kinetics and Enthalpy Measurements of Interaction between β-amyloid and Liposomes by Surface Plasmon Resonance and Isothermal Titration Microcalorimetry”, Colloids and Surfaces B: Biointerfaces, 58, 231-236, (2007) (SCI)

50. Matsuoka Y, Onodera T, Kojima T, Chen WY, Imanaka T, Fukushima H and Higuchi A*, “Novel enzymatic properties of DNA-Pt complexes”, Biomacromolecules, 8, 2684-2688, (2007) (SCI)

51. Lin YM, Liu HL*, Zhao JH, Huang CH, Fang HW, Ho Y, and Chen WY, “Molecular Dynamics Simulations to Investigate the Domain Swapping Mechanism of Human Cystatin C”, Biotechnology Progress, 23, 577-584, (2007) (SCI)

52. Chen LY, Cheng CW, Lin JJ, and Chen WY*, “Exploring the effect of cholesterol in lipid bilayer membrane on the melittin penetration mechanism”, Analytical Biochemistry, 367, 49–55, (2007) (SCI)

53. Liu HL*, Lin YM, Zhao JH, Hsieh MC, Lin HY, Huang CH, Fang HW, Ho Y, and Chen WY, “Molecular Dynamics Simulations of Human Cystatin C and Its L68Q Varient to Investigate the Domain Swapping Mechanism”, Journal Biomolecular Structure and Dynamics, 25, 135-144, (2007) (SCI)

54. Higuchi A*, Watanabe T, Noguchi Y, Chang Y, Chen WY, and Matsuoka Y, “Visible light regulates neurite outgrowth of nerve cells”, Cytotechnology, 54, 181-188, (2007) (SCI)

55. Chern CS*, Chen WY, Chiu HC, Chang FH, Hsieh MH, and Tu JR, “Interactions between amphiphilic graft copolymer-intercalated liposomes”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 277, 44-51, (2006) (SCI)

56. Lin CH, Hunag CF, Chen WY, Chang YY, Ding WH, Lin MS, Wu SH, and Huang RN*, “Characterization of the Interaction of Galectin-1 with Sodium Arsenite”, Chemical Research in Toxicology, 19, 469-474, (2006) (SCI)

57. Chen LY, Wu MC, Chou MT, Kao LA, Chen SJ, and Chen WY*, “Effects of solute-matrix interaction on monitoring the conformational changes of immobilized proteins by surface plasmon resonance sensor”, Talanta, 67, 862-867, (2005) (SCI)

58. Liu CI, Chan YC, Chen WY, and Ruaan RC, “Effect of Amino Acid Sequence on the Hydrophobicity of Small Peptides”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 266, 175-180, (2005) (SCI)

59. Liu AC, Chen LY, Chiou CF, Su HJ, Chan YC, Tasi PH, Chen SJ, and Chen WY*, “Thermodynamics and Mechanism of ssDNA Hybridization below the melting temperature by Isothermal Titration Calorimetry”, Thermochimica Acta, 433, 83-87. (2005) (SCI)

60. Chang SH, Chen LY, and Chen WY*, “The Effects of Denaturants on Protein Conformation and Behavior at Air/Solution Interface”, Colloids and Surfaces B: Biointerfaces, 41, 1-6, (2005) (SCI)

61. Chien FC, Liu JS, Su HJ, Kao LA, Chiou CF, Chen WY, and Chen SJ*, “An Investigation into the Influence of Secondary Structure on the DNA hybridization using Surface Plasmon Resonance (SPR)”, Chemical Physics Letters, 397, 429-434, (2004) (SCI)

62. Chang RS*, Lee DY, Chen WY, Huang RN, Huang HY, Hsu JH, and Lin PL, “Method for producing coded micro-carrier and test method by using a novel type biochip”, Journal of Biotechnology, 108, 1-9, (2004) (SCI)

63. Hu WP, Chen SJ*, Huang KT, Hsu JH, Chen WY, Chang GL, and Lai KA, “A novel ultrahigh-resolution surface plasmon resonance biosensor with an Au nanocluster-embedded dielectric film”, Biosensors & Bioelectronics, 19, 1465-1471, (2004) (SCI)

64. Liu DZ*, Hsieh YL, Chang SY, and Chen WY, “Microcalorimetric Studies on the Physical Stability of Polyethylene Glycol-Grafted Liposome”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 212, 227-234, (2003) (SCI)

65. Tasi LM, Liu DR, and Chen WY*, “Microcalorimetric Investigation of the Interaction of Polysorbate Surfactants with Unilamellar Phosphatidylcholine Liposomes”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 213, 7-14, (2003) (SCI)

66. Huang HM, Chen WY*, and Ruaan RC, “Microcalorimetric studies of the mechanism of interaction between designed peptides and hydrophobic adsorbents”, Journal of Colloid and Interface Science, 263, 23-28, (2003) (SCI)

67. Chen WY*, Huang HM, Lin CC, Lin FY, and Chan YC, “Effect of Temperature on Hydrophobic Interaction Between Proteins and Hydrophobic Adsorbents – Studies by Isothermal Titration Calorimetry and the van't Hoff Equation”, Langmuir, 19, 9395-9403 (2003) (SCI)

68. Lin FY, Chen WY*, and Hearn MTW, “Thermodynamic analysis of the interaction between proteins and solid surfaces: application to liquid chromatography”, Journal of Molecular Recognition, 15, 55-93, (2002) (SCI)

69. Tasi YS, Lin FY, and Chen WY*, and Lin CC, “Isothermal Titration Microcalorimetric Studies of the Effect of Salt Concentrations in the Interaction between Proteins and Hydrophobic Adsorbents”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 197, 111-118, (2002) (SCI)

70. Huang HM and Chen WY*, “Modification of Methyl Methacrylate Triblock Copolymer with Chelating Agent and Its Application in Protein Affinity Coprecipitation”, Journal of the Chinese Institute of Chemical Engineers, 33, 397-405, (2002) (SCI)

71. Chen WY*, Chen KH, Chen H, and Ruaan RC, “Effect of Block Size and Sequence on the Micellization, Hydrophobicity, and Surface Adsorption of ABC Triblock Methacrylic Polyampholytes by Isothermal Titration Calorimetry” Journal of the Chinese Institute of Chemical Engineers, 33, 599-608, (2002) (SCI)

72. Chen WY*, Lee YW, Lin SC, and Ho CW, “Renaturation and Interaction of Ribonuclease A with AOT Surfactant in Reverse Micelles”, Biotechnology Progress, 18, 1443-1446, (2002) (SCI)

73. Chen WY*, Chen CS, and Lin FY, “Molecular recognition in imprinted polymers: thermodynamic investigation of analyte binding using microcalorimetry” Journal of Chromaography A, 923(1-2): 1-6. (2001) (SCI)

74. Lin FY, Chen CS, Chen WY*, and Yamamoto S, “Microcalorimetric studies of the interaction mechanisms between proteins and Q-Sepharose at pH near the isoelectric point (pI): Effects of NaCl concentration, pH value, and temperature”, Journal of Chromatography A, 912, 281-289, (2001) (SCI)

75. Lin FY, Chen WY*, and Chen SM, “Microcalorimetric Study of the Effect of Hexa-Histidine Tag and Denaturant on the Interaction Mechanism between Protein and Metal-Chelating Gel”, Journal of Colloid and Interface Science, 238, 333-339, (2001) (SCI)

76. Lin FY, Chen WY*, and Hearn MTW*, “Microcalorimetric Studies on the Interaction Mechanism Between Proteins and Hydrophobic Solid Surfaces in Hydrophobic Interaction Chromatography: Effects of Salts, Hydrophobicity of the Gel and the Structure of the Proteins”, Analytical Chemistry, 73, 3875-3883, (2001) (SCI)

77. Lin FY, Chen WY*, Huang HM, and Ruaan RC, “Microcalorimetric Studies of Interactions between Proteins and Hydrophobic Ligands in Hydrophobic Interaction Chromatography: Effects of Ligand Chain Length, Density and the Amount of Bound Protein”, Journal of Chromatography A, 872, 37-47, (2000) (SCI)

78. Chen WY*, Kuo CS, and Liu DZ, “Determination of the Second Virial Coefficient of the Interaction between Microemulsion Droplets by Microcalorimetry”, Langmuir, 16, 300-302, (2000) (SCI)

79. Huang HM, Lin FY, Chen WY*, and Ruaan RC, “Isothermal Titration Microcalorimetric Studies of the Effect of Temperature on Hydrophobic Interaction between Proteins and Hydrophobic Adsorbents”, Journal of Colloid and Interface Science, 229, 600-606, (2000) (SCI)

80. Liu DZ and Chen WY*, Tasi LM, and Yang SP, “Microcalorimetric and shear studies on the effects of cholesterol on the physical stability of lipid vesicles”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 172, 57-67, (2000) (SCI)

81. Lin FY, Chen WY*, and Huang SY, “Selective separation of caseinophosphopeptides through immobilized metal ion affinity chromatography: effects of pH values, salt concentrations and degree of phosphorylation”, Bioprocess Engineering, 23, 467-471, (2000) (SCI)

82. Liu DZ, Chen WY*, Tsai LM, and Yang SP, “The Effects of Cholesterol on the Release of Free Lipids and the Physical Stability of Lecithin Liposomes”, Journal of the Chinese Institute of Chemical Engineers, 31, 269-276, (2000) (SCI)

83. Chen WY*, Kuo CS, and Liu DZ, “A novel method of determining the aggregation behavior of microemulsion droplets”, Progress in Biotechnology, 16, 137-141, (2000) (SCI)

84. Lin FY, Chen WY*, Ruaan RC, and Huang HM, “Microcalorimetric studies of interactions between proteins and hydrophobic ligands in hydrophobic interaction chromatography: effects of ligand chain length, density, and the amount of bound protein”, Progress in Biotechnology, 16, 59-62, (2000) (SCI)

85. Chen WY*, Kuo CS, and Liu DZ, “Microcalorimetric and Conductivity Studies of the Effects of Solutes on the Percolation Behavior of Reverse Micelle Systems”, Journal of the Chinese Institute of Chemical Engineers, 31, 455-464, (2000) (SCI)

86. Chen WY*, Dai KC, Ho KC, Lin FY, Huang HM, and Ruaan RC,” Application of Steric-Mass Action Model to Triblock Copolymer as a Displacer in Ion-Exchange Displacement Chromatography”, Journal of the Chinese Institute of Chemical Engineers, 30, 375-382, (1999) (SCI)

87. Lin FY, Chen WY*, and Sang LC, “Microcalorimetric Studies of the Interactions of Lysozyme with Immobilized Metal Ions: Effects of Ion, pH Value and Salt Concentration”, Journal of Colloid and Interface Science, 214, 373-379, (1999) (SCI)

88. Lin RY, Chen WY*, and Liao CW, “Entropy-Driven Binding/Partition of Amino Acids/Dipeptides to Stratum Corneum lipid Vesicles”, Journal of Controlled Released, 50, 51-59, (1998) (SCI)

89. Ruaan RC*, Hsu D, Chen WY, Chen H, and Lin MS, “Protein Separation by Hydrophobic Interaction Chromatography using Methacrylic Block Copolymers as Displacers”, Journal of Chromatography A, 824, 35-43, (1998) (SCI)

90. Lin RY, Ou YC, and Chen WY*, “The Role of Electroosmotic Flow n In-Vitro Transdermal Iontophoresis”, Journal of Controlled Release, 43, 23-33, (1997) (SCI)

91. Chen WY*, Lee JF, and Wu CF, and Tsao HK, “Microcalorimetric Studies of the Interactions of Lysozyme with Immobilized Cu(II): Effects of pH Value and Salt Concentration”, Journal of Colloid and Interface Science, 190, 49-54, (1997) (SCI)

92. Sung HW, Chen WY*, Tsai CC, and Hsu HL, “In vitro study of enzymatic degradation of biological tissues fixed by glutaraldehyde or epoxy compound”, Journal of Biomaterials Science: Polymer Edition, 8, 587-600, (1997) (SCI)

93. Lin RY, Hsu CW, and Chen WY*, “A Method to Predict the Transdermal Permeability of Amino Acids and Dipeptides through Porcine Skin”, Journal of Controlled Release, 38, 229-234, (1996) (SCI)

94. Chen WY*, Wu CF, and Liu CC, “Interactions of Imidazole and Proteins with Immobilized Cu(II) Ions: Effects of Structure, Salt Concentrations and pH in Affinity and Binding Capacity”, Journal of Colloid and Interface Science, 180, 135-143. (1996) (SCI)

95. Wu CF, Chen WY*, and Lee JF, “Microcalorimetric Studies of the Interactions of Imidazole with Immobilized Cu(II): Effects of pH Value and Salt Concentration”, Journal of Colloid and Interface Science, 183, 236-242, (1996) (SCI)

96. Chen FY, Chen WY*, and Kuo CS, “Protein Effect on the Bounded Water of Reverse Micelles”, Journal of the Chinese Institute of Chemical Engineers, 27, 347-351, (1996) (SCI)

97. Lin RY, Chen WY*, and Jiang TS, “In Vitro Iontrophoretic Absorption of Angiotensin II and Antagonists by Porcine Skin”, Journal of Chinese Institute of Chemical Engineers, 26, 137-145, (1995) (SCI)

98. Liu HS*, Wang YC, and Chen WY, “The sorption of lysozyme and ribonuclease onto ferromagnetic nickel powder 1. Adsorption of single components”, Colloids and Surfaces B: Biointerfaces, 5, 25-34, (1995) (SCI)

99. Chen WY*, Wang LK, and Wu CF, “Calculation of Relative Binding of Free Energy of Vancomycin with Diacetyl-L-Lysyl-D-Alanyl-D-Alanine and Diacetyl-L-Lysyl-L-Alanyl-L-Alanine”, Journal of Fermentation and Bioengineering, 80, 136-140, (1995) (SCI)

100. Wu CF, Chen WY*, and Liu HS, “Determination of the Binding Constant of Imidazole and Histidine with Immobilized Cu(II) by Differential UV Spectroscopy”, Journal of Chemical Engineering of Japan, 28, 419-423, (1995) (SCI)

101. Chen WY, Alexandrines P*, Su CK, Patrickios CS, and Hatton TA*, “Effect of Block Size and Sequence on the Micellization of ABC Triblock Methacrylic Polyampholytes”, Macromolecules, 28, 8604-8611, (1995) (SCI)

102. Lin RY and Chen WY*, “The Biological Membrane Applications of Isothermal Titration Calorimetry”, Journal of Chinese Colloid and Interface Society, 18, 119-128, (1995) (SCI)

103. Chen WY*, Liu PK, Wang LK, Wu CF, and Lin RY, “Designing Peptide by Molecular Recognition Theory as Affinity Adsorption Ligand for Peptide Purification in Affinity Chromatography”, Journal of the Chinese Institute of Chemical Engineers, 26, 19-26, (1995) (SCI)

104. Chen WY*, Wang YH, and Wang LK, “The Effect of Amino Acid Sequence on the Separation of Peptide and Protein by Reversed Micelles”, Journal of Chemical Engineering of Japan, 27, 685-687, (1994) (SCI)

105. Chen WY*, Shu CG, Chen JY, and Lee JF, “The Effect of Amino Acid Sequence on the Partition of Peptides in Aqueous Two-phase System”, Journal of Chemical Engineering of Japan, 27, 688-690, (1994) (SCI)

Conference Paper

1. W.Y. Chen “Solubility Parameters and Solution Conformation Energy Calculations for Better Peptides Purification by Liquid Chromatography” 2012 International Workshop on Environment and Resources, MCUT, New Taipei City, Taiwan, May 18 (2012)

2. Tasi CW, Hsu MY, Kao WH, and Chen W.Y.* “Exenatide Peptide Enantiomers Separation by Combing the Reversed-Phase Chromatography and Molecular Dynamics Simulation” PBAST-6, Taipei, Taiwan, May 21-24 (2012)

3. Y.C. Cheng,*, C.A. Wu , W.Y. Chen, C.J. Huang, C.C. Chien “Elucidating the Signaling Pathways of PDMCs (placenta-derived multipotent cells) Differentiated into Neuron” EuPA 2012 Conference in Glasgow, July 9 -12 (2012)

4. S. F. Lin, W. Y. Chen, and J. Y. Chang, "Metal-assisted guided-mode resonance device for biosensing,"2012 SPIE Photonic West, U.S.A. San Francisco, Jan. 21, (2012).

5. S. F. Lin, W. Y. Chen, and J. Y. Chang, "A Spectrum-free imaging guided mode resonance system for biosensing," Biosensors 2012, Mexico Cancun, May 15, (2012).

6. S. F. Lin, W. Y. Chen, and J. Y. Chang, "A Guided Mode Resonance Aptasensor for Thrombin Detection and Its Kinetics," 2nd International Conference on Bio-Sensing Technology, Netherlands Amsterdam, Oct. 10, (2011).

7. Y. H. Chu, S. F. Lin, W. Y. Chen, and J. Y. Chang, "Sensitivity Optimization of Guided Mode Resonance Biosensors," 2nd International Conference on Bio-Sensing Technology, Netherlands Amsterdam, Oct. 10, (2011).

8. Y. L. Tsai, S. F. Lin, W. Y. Chen, and J. Y. Chang, "New Image Sensor Using Optical Guided Mode Resonance," 2nd International Conference on Bio-Sensing Technology, Netherlands Amsterdam, Oct. 10 (2011).

9. Lue JH, Ding TJ, Yang TH, Chang JY, and Chen WY “Real-time Monitoring on Peptide Synthesis by GMR Biosensors” The 4th International Conference on Biomedical Engineering and Informatics (BEMI) IEEE, (2011)

10. S. F. Lin, W. Y. Chen, and J. Y. Chang," Thrombin detection using guided mode resonance aptasensor," Microoptics Conference, Shinchu, Taiwan, Oct. 31 (2010).

11. Lin SF, Yang TH, Chen WY, Liu JT, Ding TJ, Tsai YL, and Chang JY," Application of aptasensor by using guided mode resonance for thrombin detection," 7th International Conference on Optics-photonics Design & Fabrication, Yokohama, Japan, Apr. 21 (2010).

12. Wang WC, and Chen WY, "The study of solubility parameter calculations and measurements for the optimization of peptide purification by HPLC," 16th Conference on Biochemical Engineering, Tatung University, Taipei, June 24-25 (2011)

13. Wu SH, and Chen WY, "The development of Isothermal Titration Calorimetry as an emerging tool for the intermolecular interactions behavior studies of bio-molecules in the solution phase," 16th Conference on Biochemical Engineering, Tatung University, Taipei, June 24-25 (2011)

14. Lin CH, and Chen WY, "Enhancement of Mass Spectrometry Sensitivity on Phosphopeptide Using Chemical Modified Nanofilament Silicon (nSi) Chip and Iron(II, III) Oxide Magnetic Nanoparticles," 16th Conference on Biochemical Engineering, Tatung University, Taipei, June 24-25 (2011)

15. Huang LY, and Chen WY, "Optimization of DNA-conjugate Protein Chip Studies by Surface Plasmon Resonance Image," 16th Conference on Biochemical Engineering, Tatung University, Taipei, June 24-25 (2011)

16. Wu JA, and Chen WY, "Elucidate the signaling pathway of PDMCs (Placenta-Derived Multipotent Cells) differentiated into Neuron-like cells," 16th Conference on Biochemical Engineering, Tatung University, Taipei, June 24-25 (2011)

17. Lin SF, Yang TH, Chen WY, and Chang JY, "核酸適合體式光學波導共振生物感測器於凝血酶之檢測," 15th Conference on Biochemical Engineering, Southern Taiwan University, Tainan, Taiwan, June 25-26.

18. Chen WY and Lin MS, 2010, Isothermal titration calorimetry studies of real-time monitoring the heat of metabolism during PC12 cell-Aβ(1-40) interaction, 13th Asian Pacific Confederation of Chemical Engineering Congress (APCChE),Taipei, Taiwan, October 5-8. (Invited Talk)

19. Chen WY and Lin MS, 2010, Thermodynamics Studies of Alzheimer's Disease Mechanism from molecular interactions to cell, Korean Biotechnology and Bioengineering Society Annual Meeting, Seoul, Korea, April 14-16. (Invited Talk)

20. Tsai MF, Chang Y, and Chen WY, 2010, Effect of the Hydration Properties and Molecular Interaction of Sulfobetaine Polymer on Its Biocompatibility, 15th Conference for Biochemical Engineering, Southern Taiwan University, Tainan, Taiwan, June 25-26.

21. Chen RH, Lin PH, Lin ZH, and Chen WY, 2010, Studies of the Binding Mechanism between Aptamers and Thrombin by Surface Plasmon Resonance and Circular Dichroism, 15th Conference for Biochemical Engineering, Southern Taiwan University, Tainan, Taiwan, June 25-26.

22. Lin SF, Yang TH, Chen WY, and Chang JY, 2010, Guided Mode Resonance Aptasensor for Thrombin Detection, 15th Conference for Biochemical Engineering, Southern Taiwan University, Tainan, Taiwan, June 25-26.

23. Chen WY, 2009, Developments of novel optical and electrical biosensors by NEMS and its applications, Annual Meeting Chemical Society, I-Shou University, Kaohsiung, Taiwan, December 4-6. (Invited Talk)

24. Chen WY, 2009, Developments of Novel Optical and Electrical Biosensorsby NEMS and Its Applications, Asia-Pacific Biochemical Engineering Conference 2009, Kobe, Japan, Febuary 24-28. (Invited Keynote Speaker)

25. Tsao CW, Chen WY, Huang JT, Lin ZW, Chang CP, Cheng YC, and Chien CC, 2009, Fabrication of Nanofilament Silicon and its Application in Biological Mass Spectrometry, 9th Asian-Pacific International Symposium on Microscale Separations and Analysis (APCE) and 1st Asian-Pacific International Symposium on Lab on Chip (APLOC), Shanghai, China, October 28-31.

26. Chen WY, Hsu SH, and Chang Y, 2009, Studies of Proteins Adsorption Resistance of [2-(methacryloyloxy)ethyl] trimethylammonium (TMA) and 3-Sulfopropyl methacrylate potassium salt (SA) Mixed-charged Copolymer Brushs of Sulfobetain-like Surface), 14th Conference on Biochemical Engineering, National Chung Hsing University, Taichung, Taiwan, June 5-6.

27. Chen WY, Yu SS, and Chang Y, 2009, Studies of surface grafting control and characterization of zwitterionic polymer brushes on tissue cultural polystyrene plates and of their anti-bacterial properties, 14th Conference on Biochemical Engineering, National Chung Hsing University, Taichung, Taiwan, June 5-6.

28. Lin MS and Chen WY, 2008, Kinetics and Thermodynamics Analyses of Biomolecular Interaction, Bioseparation for Biorecognition and Bionanotechnology Conference, Souel, Korea, October 27-28. (Inivtied Lecture)

29. Chen WY, 2008, The Thermodynamics Studies of Biomacromolecular Interactions and Its Application in Bioseparation, Japan Polymer Society, Tokyo, Japan, September 24-25. (Invited Plenary Speaker)

30. Lin MS, Tsai HT, and Chen WY, 2008, Investigation of the Mechanism of β-amyloid Fibril Formationby Kinetic and Thermodynamic Analyses, 13th Biochemical Engineering Conference, Taoyuan, Taiwan, June 27-28. (The Outstanding English Oral Paper Award)

31. Tang ST and Chen WY, 2008, The enantiomeric separation by using DNA aptamers, 13th Biochemical Engineering Conference, Taoyuan, Taiwan, June 27-28. (The Outstanding poster paper Award)

32. Lin PS, Liu JT, and Chen WY, 2008, A novel method for detecting microalbuminuria by surface plasmon resonance, 13th Biochemical Engineering Conference, Taoyuan, Taiwan, June 27-28.

33. Liao SC, Ding TJ, Yang TH, Chen WY, Liu JT, Lee CC , and Chang,JY, 2008, Optical Label-Free Gene-Chip Applying Guided-Mode Resonance Efficiency, 13th Biochemical Engineering Conference, Taoyuan, Taiwan, June 27-28.

34. Su CP, Chen WY, and Higuchi A, 2008, Biocompatible studies of plasma protein adsorption and platelet adhesion on porous polymeric membranes, 13th Biochemical Engineering Conference, Taoyuan, Taiwan, June, 27-28.

35. Siao YD, Higuchi A, Chang Y, and Chen WY, 2008, Preparation of a DNA aptamer-Pt Complex and its Use in the Colorimetric Sensing of Thrombin and Anti-thrombin Antibodies, 13th Biochemical Engineering Conference, Taoyuan, Taiwan, June, 27-28.

36. Liao SC, Chang Y, and Chen WY, 2008, A highly Nonbiofouling Surface with Well-Packed Grafted Zwitterionic Polysulfobetaine for Plasma Protein Repulsion, 13th Biochemical Engineering Conference, Taoyuan, Taiwan, June, 27-28.

37. Yang ST, Chen WY, and Higuchi A, 2008, Separation and Purification of Hematopoietic Stem Cells from Human Blood through Surface-modified Membranes, National Central University (NCU) – Tokyo Institute Technology (TIT) International Student Visiting Program, June 22-30.

38. Hsu SH, Chen WY, Chang Y, and Higuchi A, 2008, Protein adsorption on the modification surface using Nano-segment polymer, National Central University (NCU) – Tokyo Institute Technology (TIT) International Student Visiting Program, June 22-30.

39. Lee JH, Chen WY, and Higuchi A, 2008, Stabilization Bioassay for FGF-1 and EGF, National Central University (NCU) – Tokyo Institute Technology (TIT) International Student Visiting Program, June 22-30.

40. Ho LY, Ling QD, Chen WY, and Akon Higuchi, 2008, Regulation of Neurite Outgrowth of Nerve Cells by Irradiation of Light, National Central University (NCU) – Tokyo Institute Technology (TIT) International Student Visiting Program, June 22-30.

41. Yu SH, Chen WY, and Higuchi A, 2008, Preservation and Cultivation of Stem Cells on Surface-modified Membranes having Nano-segments (Pluronic), National Central University (NCU) – Tokyo Institute Technology (TIT) International Student Visiting Program, June 22-30.

42. Hung SY, Chen WY, and Higuchi A, 2008, Purification and Cultivation of Mesenchymal Stem Cells through Surface-modified Membranes, National Central University (NCU) – Tokyo Institute Technology (TIT) International Student Visiting Program, June 22-30.

43. Su CP, Yu SH, Chen WY, Chang Y, and Higuchi A, 2008, Preservation of Hematopoietic Stem Cells on Pluronic-immobilized Surface, 2008 World Biomaterials Congress, Amsterdam, Netherland, May 28-Jun 1.

44. Siao YD, Fukushima H, Higuchi A, and Chen WY, 2008, Preparation and Characterization of DNA-Pt Complexes as Novel Enzymatic Prpperties, 2008 World Biomaterials Congress, Amsterdam, Netherland, May 28-Jun 1.

45. Yang ST, Chen WY, Chang Y, and Higuchi A, 2008, Separation and Purification of Hematopoietic Stem Cells from Human Blood through Surface-modified Membranes, 2008 World Biomaterials Congress, Amsterdam, Netherland, May 28-Jun 1. (International Student Travel Award)

46. Lin PH, Yen SL, Higuchi A, and Chen WY, 2008, Microcalorimetrics Studies of the Thermodynamics and Binding Mechanism between L-Tyrosinamide and Aptamer, 14th Symposium on Chemical Thermodynamics and Thermal Analysis, Dalian, China, May 21-25. (Invited Speaker)

47. Chen WY, 2008, Microcalorimetric Studies on the Interaction Mechanism between Proteins and Hydrophobic Solid Surfaces in Hydrophobic Interaction Chromatography: Effects of Salts, Hydrophobicity of the Sorbent, and Structure of the Protein, The 5th International and 7th China-Japan Joint Symposium on Calorimetry and Thermal Analysis and Exhibition, Dalian, China, May 18-21. (Invited Speaker)

48. Liu JT, Lin PS, and Chen WY, 2008, A novel surface modification method for the detection of microalbuminuria by surface plasmon resonance, the Tenth World Congress on Biosensors, Shanghai, China, May 14-16.

49. S. F. Lin, Z. C. Liau, T. H. Yang, W. Y. Chen, J. T. Liu, T. J. Ding, Y. L. Tsai, and J. Y. Chang," Real Time Label-Free Gene Chip Base on Guided Mode Resonance," International Conference on Laser Applications in Life Sciences.

50. Liu JT, Lin PS, and Chen WY, 2007, Surface plasmon resonance detection of Human Serum Albumin by a novel method, 2007 International Symposium on Biomedical Engineering (2007生物醫學工程科技研討會暨國科會醫學工程學門成果發表會) ,Taichung , Taiwan, December 14-15. (The Outstanding poster paper Award)

51. Liu ZC, Shih MC, and Chen WY, 2007, Using the Surface Plasma Resonance Biosensor for Glycated- hemoglobin Detection, The Asia-Pacific Biochemical Engineering Conference, Taipei, Taiwan, November 4-7.

52. Tsai JZ, Liu ZC, Chen CJ, Hsueh KH, Hsin YM, and Chen WY, 2007, Characterization of Interdigitated Electrode Pairs as Biosensors for Human Serum Albumin, The Asia-Pacific Biochemical Engineering Conference, Taipei, Taiwan, November 4-7. (The Outstanding poster paper Award)

53. Lin MS and Chen WY, 2007, Examining the levels of ganglioside and cholesterol in cell membrane on attenuation the cytotoxicity of beta-amyloid peptide, The Asia-Pacific Biochemical Engineering Conference, Taipei, Taiwan, November 4-7.

54. Higuchi A, Chang Y, Ruaan RC, and Chen WY, 2007, Separation of hematopoietic stem cells from human peripheral blood through surface-modified polyurethane membranes, The Asia-Pacific Biochemical Engineering Conference, Taipei, Taiwan, November 4-7.

55. Chiag YC, Ruaan RC, Chen WY, and Chang Y, 2007, Low Fouling Poly(vinylidene fluroride) UF Membranes with Grafted Zwitterionic Sulfobetaine for Proteins Separation, The Fourth Conference of Aseanian Membrane Society, Taipei, Taiwan, August 16-18.

56. Shih YJ, Ruaan RC, Chang Y, and Chen WY, 2007, Bio-fouling Property of Poly(vinylidene fluroride) Membrane with Surface-Copolymerized Poly(ethylene glycol) Methacrylate, The Fourth Conference of Aseanian Membrane Society, Taipei, Taiwan, August 16-18.

57. Lin M, Fan FJ, and Chen WY, 2007, The Kinetics and Thermodynamics Studies of b-Amyloid Interactions with Liposome by Surface Plasmon Resonance and Isothermal Titration Calorimetric, 2007 Current Trends in Microcalorimetry, Boston, USA, July 19-21.

58. Lin MS, Tsia HT, and Chen WY, 2007, Investigation the Mechanism of b-Amyloid Fibrils Formation by Kinetics and thermodynamics Analysis, 2007 Current Trends in Microcalorimetry, Boston, USA, July 19-21.

59. Chen WY, 2007, Isothermal Titration Calorimetry as a tool for analyzing the thermodynamics information of biochromatography, VIT-Biochromatography and Nanotechnologies 2007, Vellore, India, February 11-15. (Invited speaker)

60. Hsu CH, Chieh LS, and Chen WY, 2007, Isothermal Titration Calorimetric Studies of the Non-Fouling Interaction Mechanism between Proteins and Polyethylene Glycol, The 12th Biochemical Engineering Conference (第十二屆生化工程研討會), Taoyuang, Taiwan, June 29-30.

61. Hsu CH, Chieh LS, and Chen WY, 2007, Studies of the Binding Thermodynamics and Conformation between Aptamer and Analyte by Isothermal Titration Calorimetric and Circular Dichroism, The 12th Biochemical Engineering Conference (第十二屆生化工程研討會), Taoyuang, Taiwan, June 29-30.

62. Chen HP, Lin MS, and Chen WY, 2007, Interaction Mechanism Studies between b-Amyloid and Lipid Monolayer by Fluorescence Microscopy and Langmuir-Blodgett Trough, The 12th Biochemical Engineering Conference (第十二屆生化工程研討會), Taoyuang, Taiwan, June 29-30.

63. Lin MS, Tsai HT, and Chen WY, 2006, Kinetics and Thermodynamics Analysis the Mechanisms of β–Amyloid Fibrils Formation, YABEC 2006 Symposium, Kaohsiung, Taiwan, November 25-27.

64. Liu ZT, Shih MC, Yen SL, and Chen WY, 2006, A Surface Plasmon Resonance Biosensor for Glycated-Hemoglobin Detection, YABEC 2006 Symposium, Kaohsiung, Taiwan, November 25-27.

65. Tasi JZ, Chen CJ, Hung CK, Chen WY, and Hsin YM, 2006, Study of the Impedance Sensor Array for DNA Detection by Hybridization with Au-Nanoparticles, YABEC 2006 Symposium, Kaohsiung, Taiwan, November 25-27.

66. Kim KH, Lin MS, Chen WY, and Lee EK, 2006, Analysis of BSA Adsorption to Aluminum Hydroxide Gel by using ITC, DSC, and Equilibrium Isotherm, YABEC 2006 Symposium, Kaohsiung, Taiwan, November 25-27.

67. Chang YW, Hsieh YJ, Chen WY, and Tsai HH, A long time scale convenient molecular dynamics simulations of the folding of met-enkephalin in explicit water and on membrane, 2006 Annual Meeting of the Chinese Chemical Society (中國化學會2006年年會), Tamsui, Taiwan, November 24-26.

68. Chen WY, 2006, Isothermal Titration Calorimetry as a Tool for Analyzing the Thermodynamics Aspects of Chromatography of Proteins and DNAs, 6th Ube international symposium on bioseparation and biorecognition engineering, Ube, Japan, November 23-25. (Invited Speaker)

69. Hu WP, Su YD, Chen SJ, and Chen WY, 2006, Multipoints Measurement of DNA Hybridization with a Common-path Phase-shift (PSI) Surface Plasmon Resonance, 5th Asia-Europe Biorecognition Engineering Conference, Vienna, Austria, October 21.

70. Liu JT, Tasi PS, Lin MS, Shu CS, Yamamoto S, and Chen WY, 2006, Studies of the Interaction Mechanism between single strand and double strand DNA with Hydroxyapatite by Microcalorimetry and Isotherm Measurements, International Separations for Proteins, Peptides, and Polynucleotides, Innsbruck, Austria, October 17-20.

71. Fan FJ,Lin MS, Chen SB, and Chen WY, 2006, Kinetics studies of interactions between b-amyloid and various composition liposome by surface plasmon resonance, The 11th Biochemical Engineering Conference (第十一屆生化工程研討會), Hsinchu, Taiwan, June 23-24.

72. Tsai HT, Lin MS, Chen SB, and Chen WY, 2006, Kinetics and thermodynamics studies of β-amyloid peptide aggregation in aqueous solution, The 11th Biochemical Engineering Conference (第十一屆生化工程研討會), Hsinchu, Taiwan, June 23-24.

73. Hu WP, Su YD, Chen SJ, Chen WY, Chang GL, Taylor A, and Jiang S, 2006, Multipoint Measurement of DNA Hybridization with a Common-path Phase-shift Interferometry Surface Plasmon Resonance Imaging System, Biosensors 2006, Toronto, Canada, May 10-12.

74. Yih JN, Chiu KC, Chien FC, Chen WY, and Chen SJ, 2006, An investigation into the influence of secondary structures for DNA hybridization using surface plasmon resonance (SPR) and surface-enhanced Raman scattering (SERS), Photonics West 2006, San Jose, California, USA, Jan 21-26.

75. Liu JT, Shih MC, Lin CW, Yen SL, Siao YD, and Chen WY, 2005, A Boronic Acid-based Quartz Crystal Biosensor for Sugar and Glycated hemoglobin detection, Nano Bioengineering International Symposium -- Quantum Dots for Live Cells, in vivo Imaging, and Diagnostics (2005奈米生物工程國際研討會--量子點在細胞活性監測、生醫光電影像及臨床診斷之應用), Taoyuang, Taiwan, December 15-16.

76. Wu JH, Huang HC, Hsu CW, Yang TH, Chang JY, and Chen WY, 應用波導共振光學元件之新型生物感測器, Optics and Photonics Taiwan’05 (2005年台灣光電科技研討會), Tainan, Taiwan, December 9-10.

77. Chiu HM and Chen WY, 2005,利用表面電漿共振儀及恆溫滴定微卡計探討微脂粒與類澱粉胜肽交互作用之動力學及熱力學研究, The 10th Biochemical Engineering Conference (第十屆生化工程研討會),Taipei, Taiwan, June 24-25.

78. Tsai PH and Chen WY, 2005,利用恆溫吸附曲線及恆溫滴定微卡計探討單股及雙股DNA 與Hydroxyapatite間交互作用之機制與熱力學, The 10th Biochemical Engineering Conference (第十屆生化工程研討會),Taipei, Taiwan, June 24-25.

79. Wang CH, Tsai JZ, and Chen WY, 2005,單股DNA 於微奈米溝槽電極之基因晶片上固定化與雜交效率之最佳化, The 10th Biochemical Engineering Conference (第十屆生化工程研討會),Taipei, Taiwan, June 24-25.

80. Chung YL, Chen WY, Yamamoto S, and Chiang AST, 2005, The implementation of Gradient Elution IEC in SMB system, Japan Separation Process Engineering Society Annual Meeting, Osaka , Japan , June 3-4.

81. Chen WY, Wang CH, Huang HC, Yang TH, Chang JY, and Tsai JZ, 2005, Monitoring of ssDNA hybridization by surface plasmon resonance, guided-mode resonance, and nanogap electrode biosensor, 4th Asia-Europe Biorecognition Engineering Society, Ansan, Korea, May 13-15.

82. Chen WY, Huang HM, Lin CC, Lin FY, and Chan YC, 2004, The Effect of Temperature on Hydrophobic Interaction Between Proteins and Hydrophobic Adsorbents – Studies by Isothermal Titration Calorimetry and van't Hoff Equation, Bioseparation, Fukuoka, Japan, October 15-17.

83. Chen WY, Kao LA, and Liu JS, 2004, Examples of Thermodynamics and Kinetics Studies of Biorecognition Processes by Isothermal Titration Calorimetry and Surface Plasmon Resonance, 3rd Europe-Asia Biorecognition Engineering Conference, Shishimonoseki, Japan, September 25-26.

84. Liang BR, Syu SJ, Tasi TL, Hsu JH, and Chen WY, 2004, Au Nanoparticle-enhanced Electrical Detection of DNA Hybridization on Silicon Chips, Taiwan International Conference on Nano Science and Technology, Taipei ,Taiwan, June 30- July 3.

85. Chen WY and Kao LA, 2004,以表面電漿共振儀研究單股去氧核醣核酸之二級結構於去氧核糖核酸雜交在動力學與反應機制的效應, The 9th Biochemical Engineering Conference (第九屆生化工程研討會), Chenghua, Taiwan, June 25-26.

86. Chen WY and Wu MC, 2004,利用表面電漿共振生物感測器及單分子模型槽研究蛋白質在固/液及氣/液界面上之結構, The 9th Biochemical Engineering Conference (第九屆生化工程研討會), Chenghua, Taiwan, June 25-26.

87. Chen WY and Lin JJ, 2004,穿膜胜肽與生物細胞膜間的交互作用之探討(Ι)-膽固醇的含量對蜂毒胜肽穿膜機制之影響, The 9th Biochemical Engineering Conference (第九屆生化工程研討會), Chenghua, Taiwan, June 25-26

88. Chen WY, Kao LA, Chen SJ、Su HJ、Chio CF, 2004, Surface Plasmon Resonance配合分子模擬於核酸分子雜交反應條件最佳化之研究, The 10th Chemical Sensor Technology Conference (第十屆化學感測器科技研討會), Taipei, Taiwan, May 29.

89. Chen WY, Tao CJ, Lin JJ, Chiou SM, and Tasi PH, 2004, Studies of Interactions between Penetration Peptides with Cell Membranes (I) - The Effects of Cholesterol Content on Melittin Interactions with Lipid Membrane, The 9th Liposome research conference, Hsinchu, Taiwan, May 11-15.

90. Liang BR, Syu SJ, Tasi TL, Hsu JH, Hsin YM, and Chen WY, 2003,以奈米金粒為標記驗證DNA在二氧化矽,九十二年度中華民國醫學工程年會暨研討會, Taipei, Taiwan, December 13.

91. Chen SJ, Huang CH, Chen WY, Chang GL, Lai KA, Hu WP, 2003,超高解析度表面電漿生醫感測器, 醫學工程學會/研討會, Taipei, Taiwan, September, 2003

92. Liu AC and Chen WY, 2003,利用恆溫滴定微卡計於核酸分子雜交反應之熱力學與機制的研究, The 8th Biochemical Engineering Conference (第八屆生化工程研討會), Kaohsiung, Taiwan, June 27-28.

93. Hsu JH and Chen WY, 2003,金奈米粒子表面上自我聚集單層膜之改質及其在生物分子作用上的應用, The 8th Biochemical Engineering Conference (第八屆生化工程研討會), Kaohsiung, Taiwan, June 27-28.

94. HM Huang, Chen WY, and Ruaan RC, 2002, Microcalorimetric Studies of the Hydrophobic Interaction Between Designed Peptides and Hydrophbic Adsorbents, YABEC 2002 Symposium, Taipei, Taiwan, November 10-12.

95. Chen WY, 2002, Applications of Isothermal Titration Calorimetry in the Studies of Biological Related Nanoparticles and Nano-Structure Materials, International Bionanotechnology Conference, Taipei, Taiwan, September 5-6. (Invited)

96. Fang CI and Chen WY, 2002,利用微卡計對於蛋白質與離子交換樹脂觸手間親疏水作用力的研究, The 7th Biochemical Engineering Conference (第七屆生化工程研討會), Taipei, Taiwan, June 28-29.

97. Chen WY, Ho YC, and Liu DZ, 2002,三團聯聚合物(PF-127)和磷脂質間交互作用力量測和磷脂質微脂粒穩定度之探討, The 7th Biochemical Engineering Conference (第七屆生化工程研討會), Taipei, Taiwan, June 28-29.

98. Chen WY and Chang SH, 2002,蛋白質在氣/液界面上的行為研究與其在蛋白質復性上的應用, The 7th Biochemical Engineering Conference (第七屆生化工程研討會), Taipei, Taiwan, June 28-29.

99. Chen WY, Chou MT, and Chen LY, 2002,不同化學變性劑、溫度和還原劑對溶菌結構改變之研究, The 7th Biochemical Engineering Conference (第七屆生化工程研討會), Taipei, Taiwan, June 28-29.

100. Chen WY and Lin CC, 2001, The effect of Temperature on Hydrophobic Interaction between Proteins and Hydrophobic Adsorbents, The Sixth Biochemical Engineering, Taiwan, R.O.C., National Central University, Jhongli, Taiwan, June 30.

101. Chen WY and Lee YW, 2001, Refolding and Interfacial Phenomena of Ribonuclease A in AOT Reverse Micelle, The Sixth Biochemical Engineering, Taiwan, R.O.C., National Central University, Jhongli, Taiwan, June 30.

102. Chen WY and Ho YT, 2001, The Studies of Physical Stability and Interaction Mechanism Between Liposomes: Effects of the PEO-PPO-PEO Tri-Blocl copolymer and Mg++ Ions, The Sixth Biochemical Engineering, Taiwan, R.O.C., National Central University, Jhongli, Taiwan, June 30.

103. Chen WY, Chen CS, and Lin FY, 2000, Microcalorimetric Studies of the Binding Mechanism of 2,4-Dichlorophenoxyacetic acid by Imprinting Polymer of 4-Vinylpyridine, International Conference on Bioseparation Engineering, Ube, Japan, July 21.

104. Chen WY, Lin FY, and Huang HM, 1999, Microcalorimetric Studies of the Interaction Proteins with Hydrophobic Ligand in Hydrophobic Interaction Chromatography: Effects of Ligand Chain Length, Density and Amount of Protein Bounded, International Conference on Bioseparation Engineering, Nikko, Japan, July 4-7.

105. Chen WY, Kuo CS, and Liu DZ, 1999, Determination of the Second Virial Coefficient of the Interaction between Microemulsion Droplets by Microcalorimetry, International Conference on Bioseparation Engineering, Nikko, Japan, July 4-7.

106. Liu DZ, Chen WY, Tasi LM, and Yang SP, 1999, Microcalorimetric Studies of the Effects of Cholesterol on the Pyhsical Stability of Liposome, 4th Biochemical Engineering Conference, National Chung-Cheng University, Chiayi, Taiwan, June 27-28.

107. Tasi LM and Chen WY, 1999, The effects of Polysorbate Surfactant on the Physical Stability and the Solute’s Permeability of Liposome, 4th Biochemical Engineering Conference, National Chung-Cheng University, Chiayi, Taiwan, June 27-28.

108. Huang HM and Chen WY, 1999, Modification of Methly Methacrylate Triblock Copolymer with Chealting agent and It’s Applications in Protein Affinity Coprecipitation, 4th Biochemical Engineering Conference, National Chung-Cheng University, Chiayi, Taiwan, June 27-28.

109. Lin FY, Chen WY, and Ruaan RC,1999, Microcalorimetric Studies of the Interactions of Proteins with Hydrophobic Ligand in Hydrophobic Interaction Chromatography: Effects of Ligand Chain Length, Density and Amount of Bounded Protein, The Third Taipei-Kyushu Joint Symposium on Chemical Engineering, Taipei, Taiwan, March 2-3.

110. Chen WY, 1998, The Solution Behavior of Triblock Copolymer and Its’s Application in Ion-Exchange Displacement Chromatography, YABEC’98, Hualien, Taiwan, November 28-30.

111. Chen WY, 1998, Study of the Application of Immobilized Metal Ion Affinity Displacement Chromatography, YABEC’98, Hualien, Taiwan, November 28-30.

112. Chen WY and Lin FY, 1998, Microcalorimetric Studies of the Interaction of Lysozyme with Immbolized Fe(III): Effects of pH Value and Salt Concentration, 18th International Symposium on Separation of Proteins, Peptides and Polynucleotides, Vienna, Austria, November 1-4.

113. Chen WY and Wu CF, 1996, Microcalorimetric Studies of the Interaction of Immobilized with Immobilized Cu(II): Effects of pH value and Salt Concentration, 10th International Biotechnology Symposium, Sydney, Australia, August 25-30.

114. Chen WY and Lin RY,1996, Thermodynamic of Amino Acid and Dipeptides Binding to Stratum Corneum Lipid Liposomes10th International Biotechnology Symposium, Sydney, Australia, August 25-30.

115. Chen WY, Wu CF, and Liu CC,1996, Interactions of Imidazole and Proteins with Immobilized Cu(II) Ions: Effects of Structure, Salt Concentrations and pH in Affinity and Binding Capacity, 7th Congress of Asian Pacific Confederation of Chemical Engineers, Taipei, Taiwan, March 4-6.

116. Wu CF and Chen WY, 1995, Determination of the Binding Constant of Imidazole and Histidine with Immobilized Cu(II) by Differential UV Spectroscopy, 1995 International Chemical Congress of Pacific Basin, Hawaii, USA ,December 17-22.

117. Chen WY and Ou YC, 1995, The Role of Electroosmotic Flow and Drugs Hydrophobicity in Transdermal in Vitro, The 3rd US-Japan Symposium on Drug Delivery Systems, The Westin Maui, Maui, Hawaii, USA, December 17-22.

118. Chen WY and Young SL, 1995, The Application of Hydrogel in Drug Controlled Release - Transport and Interaction of Amino Acids in Hydrogel, The 10th World Congress of the International Society for Artificial Organs, Taipei, Taiwan, November 14-18.

119. Wu CF, and Chen WY, 1995, Determination of the Binding Constant of Imidazole and Histidine with Immobilized Cu(II) by Differential UV Spectroscopy, American Chemical Society, Anaheim, CA, USA, April 2-6.

120. Chen WY, Wang LK, and Wu CF, 1994, Calculations of Relative Binding Free Energy of Vancomycin with Diacetyl-L-Lysyl-D-Alanyl- D-Alanine and Diacetyl-L-Lysyl-L-Alanyl-L-Alanine, International Minisymposium of Bioseparation Engineering, Kyoto, Japan, September 27-29. (Invited Specker)

121. Li TL, Lee SJ, Chen WY, Kildsig DO, and Park K, 1994, Computer Simulation for Drug Diffusion in Amophorus Polymer, International Controlled Release Conference, Nice, France, June 27-30.

122. Chen WY and Wu CF, 1994, The Adsorption Behavior of Proteins by Immobilized Metal Ion and the Determination of Exposed Histidine of Protein by Molecular Modeling, 3rd Asia-Pacific Biochemical Engineering Conference, Singapore, June 13-15.

Book

1. Chu IM and Chen WY, “Partition of Amino Acids and Peptides in Aqueous Two-Phase Systems”, Methods in Biotechnology, Hatti-Kaul R (Ed), Humana Press, Volume 11, Chapter 9: 95-105, 2000.

2. Chen WY and Lin FY, “Microcalorimetry of Biomaterials at Interface”, Interfacial Dynamics, Kelley N (Ed), Surfactant Science Series, Marcel Dekker, Volume 88, Chapter 11: 419-433, 2000.

3. Chen WY and Fu-Yung Lin, “Microcalorimetry of Solid Surfaces”, Encyclopedia of Surface and Colloid Science, Hubbard AT (Ed), Marcel Dekker, P: 3402 – 3412, 2002.
研究計畫區
本研究室之研究重點為探討生物分子辨識系統中生物子之交互作用之熱力學與動力學。我們發展了恆溫滴定卡計(ITC)及藉著奈米粒子以強化之表面電漿共振儀(SPR)來量測生物分子作用之作用焓與動力學資訊。配合電腦分子模擬及表面拉曼光譜,我們可以建立一完整的熱力學、動力學及分子結構之行為以了解生物分子間作用之機制。這所建立之資訊提供了生物奈米、藥物設計、蛋白質工程及生化分離等領域之基本知識。本研究室正執行及有興趣之研究計劃為(1)蛋白質摺疊及致病之可能機制之研究:藉由逆微胞、微脂粒及固態表面等提供不同介面特性利用SPR、CD、螢光光譜儀來量測蛋白質分子之結構及聚集行為,配合活性量測來探討蛋白質─蛋白質或蛋白質─細胞膜之交互作用行為來提供蛋白質結構相關疾病(如滋海默症)之致病機制。(2)DNA雜交機制之研究:利用ITC及SPR並設計多種DNA以了解DNA雜交之熱力學與動力學,以提供基因晶片業者於探針之設計與晶片操作條件之設計。(3)利用本研究之成果延伸至對DNA或RNA核適體與胺基酸或胜肽之作用機制,除了提供系統生物學上於基因進化中有關riboswitch之解釋外,本研究也利用核適體之於特定生物分子之結合親和性,並配合DNA-Pt複合物之生物酵素,期望建立一新檢測系統。(4)生醫材料表面之改質:利用SPR及ITC之基礎量測說明生醫材料表面之改質(如PEG等)與蛋白質吸附及所誘發之細胞吸附行為之探討。相關之應用研究領域包括PEG及三團聯共聚物於生醫材料表面改質於幹細胞之純化與培養之應用。以上研究皆利用ITC與SPR等相關設備,這些設備之整合應可提供蛋白質體等研究之技術平台。
專利區
1. 陳文逸、李文仁、張榮森、黃榮南、許景翔, 生物晶片之製造方法及其應用, 中華民國專利(申請號碼91120544), 中華民國 發明專利公告號:200622244 (2006/07/01)

2. 陳文逸、許景翔, 檢驗甲醇的方法與甲醇檢測載體的製作方法, 中華民國專利(申請號碼92113287), 中華民國 發明專利公告號:I249030 (2006/02/11)

3. 陳顯禎、林俊佑、簡汎清、黃崑財、胡文品、陳文逸、李冠卿、李文獻, 一種高靈敏度表面電漿共振感測器, 中華民國專利 (申請號碼921014837), 中華民國 發明專利公告號:586005 (2004/05/01)

4. Chen SJ, Lin GY, Chien FC, Hu WP, and Chen WY, A nanoparticle-enhanced ultrahigh-sensitivity surface plasmon resonance biosensor, U.S. Patent submitted on March, 2003.11.

5. 陳顯禎、蘇園登、黃崑財、易政男、施至鴻、李文獻、李冠卿、陳文逸, 攜帶式表電漿共振與表面強化拉曼散射光電系統及其感測方法, 中華民國 發明專利公告號:I229734 (告號日期:2004/03/01)

6. 辛裕明、蔡章仁、陳文逸, 偵測核酸雜交反應的生物晶片與偵測蛋白質的生物晶片及其偵測方法, 中華民國 發明專利公告號:I293115 (告號日期:2008/2/1)

7. 曹嘉文、陳文逸, 一種具有分離、質譜轉移與質譜檢測功能的新型分析晶片, 中華民國 發明專利 (申請中)

8. 曹嘉文、陳文逸, 一種高產量、迅速且低成本的奈微米結構製造技術, 中華民國 發明專利 (申請中)

9. Chang Y, Ruaan RC, Chen WY, Higuchi A, Chaing YC, Lai JY ” Low biofouling filtration membranes and their forming method” USA, US8062751B2(2011/11/22)

10. 陳文逸、蕭伊迪、樋口亞紺” ㄧ種利用核適體與核適體-鉑複合物於分子檢測之新方法” 中華民國, (申請中)

11. Chang JY, Chen WY, Chien CC, Lin SF “Metal Buffer Layer Assisted Guided Mode Resonance Biosensor”USA, (申請中)

12. 張正陽、陳文逸、簡志誠、林聖富.“具金屬緩衝層之波導共振生物感測器” 中華民國, (申請中)

13. Chen WY, Chang LC, Ruaan RC “Peptide Chromatographic Purification Assisted by Combining of Solubility Parameter and Solution Conformation Energy Calculations” USA, (Patent Application, 2012)

14. Chang L-C and Chen WY,. An Efficient Chromatographic Method for Purification. (United States Provisional Patent Application, 2011)

15. 張雍, 阮若屈, 陳文逸, 樋口亞紺,江衍徹,賴君義; “低結垢含氟過濾膜及其形成方法

Low biofouling filtration membranes and their forming method”, 美國專利, 11/963,406 (申請日期2007年12月21日; 專利獲準2011年11月22日「證書號 US 8,062,751 B2」); 台灣專利, 97105269 (申請日期2008年2月15日)

16. 張雍, 陳文逸; “抗生物結垢非離子型-兩性離子型共聚物/Antibiofouling nonionic-zwitterionic copolymer”, 美國專利, 12/648,761 (申請日期2009年12月29日); 台灣專利, 098145931 (申請日期2009年12月30日) ; 中國專利, 201010623238.1 (申請日期2009年12月28日)

17. 張雍, 阮若屈, 江衍徹,陳文逸 ; “抗生物分子沾黏塗佈劑設計方法”, 美國專利 (預計申請日期2012年3月); 台灣專利 (預計申請日期2012年4月)

18. 張雍, 阮若屈, 江衍徹,陳文逸“抗生物沾黏之塗佈組成物、抗生物沾黏薄膜及抗生物沾黏薄膜之製造方法”in preparation 宇騰國際