專任教師-系統生物與生物資訊組   首頁 師資介紹 專任教師-系統生物與生物資訊組
陳健生 教授 Chien-Sheng Chen
從聘系所:
專線電話:03-4227151轉36103, 36105
傳真電話:
學經歷

2013 -        PLoS One - Academic Editor

2013 -        Journal of Integrated OMICS - Associate Editor

2012 -        National Central University - Associate Professor

2008 - 2011   National Central University - Assistant Professor

2008 - 2012   Department of Health, Executive Yuan, Taiwan - Food Safety

       Consultant

2007 - 2008   National Taiwan Ocean University - Assistant Professor

2005 - 2007   Johns Hopkins University - Postdoctoral fellow

2001 - 2005   Cornell University - Ph.D.

1996 - 1998   National Taiwan University - M.S.

1992 - 1996   National Taiwan Ocean University - B.S.



論文著作區

 

1.          Chen P-C, Syu G-D, Chung K-H, Ho Y-H, Chung F-H, Chen P-H, Lin J-M, Chen Y-W, Tsai S-Y*, and Chen C-S* (2015) Antibody Profiling of Bipolar Disorder using Escherichia coli Proteome Microarrays Molecular & Cellular Proteomics 14(3):510-8. (IF: 7.254) 指導博士後為第一、第二作者;指導學生為第四、第七、第八作者

2.          Chen Y-W, Teng C-H, Ho Y-H, Ho T-Y J, Huang W-C, Hashimoto M, Chiang I-Y, and Chen C-S* (2014) Identification of bacterial factors involved in type 1 fimbria expression using an Escherichia coli K12 proteome chip. Molecular & Cellular Proteomics 13(6):1485-94. (IF: 7.254) 指導學生為第一、第三及第四作者

3.          Sutandy FXR, F S-H Hsiao, Chen C-S* (2014) High throughput platform to explore RNA-protein interactomes. Critical Reviews in Biotechnology 15:1-9. (IF: 7.837) 指導學生為第一作者;指導博士後為第二作者

4.          Fan BLu K-YSutandy F.X.R., Chen Y-WKonan KZhu HKao C-C*, Chen C-S* (2014) A human proteome microarray identifies that the heterogeneous nuclear ribonucleoprotein K (hnRNP K) recognizes the 5' terminal sequence of the hepatitis C virus RNA. Molecular & Cellular Proteomics 13(1):84-92. (IF: 7.254) 指導學生為共同第一作者及第三、第四作者

5.          Ho T-Y J, Chan C-C, Chan K, Wang Y-C, Lin J-T, Chang C-M, Chen C-S* (2013) Development of a Novel Bead-based 96-well Filtration Plate Competitive Immunoassay for the Detection of Gentamycin. Biosensors & Bioelectronics 15;49:126-32. (IF: 6.451) 指導學生為第一、第二及第五作者

6.          Sutandy FXR, Qian J, Chen C-S* and Zhu H* (2013) Overview of Protein Microarrays. Current Protocols in Protein Science Chapter 27:Unit 27.1. 指導學生為第一作者

7.          Yeh S-B, Chen C-S, Chen W-Y, Huang C-J* (2014) Modification of Silicone Elastomer with Zwitterionic Silane for Durable Antifouling Properties. Langmuir 30(38):11386-93. (IF: 4.384)

8.          Su W-H, Ho T-Y, Tsou T-H, Lee W-L, Wang K-C, Yu Y-Y, Chen T-J, Tan C-H, Kuo C-D, Chen C-S*, Wang P-H* (2013) Development of a chip-based multiplexed immunoassay using liposomal nanovesicles and its application in the detection of pathogens causing female lower genital tract infections. Taiwanese Journal of Obstetrics & Gynecology 52(1):25-32. (IF: 1.261) 指導學生為第一、第二作者

9.          Wang Z-X, Deng R-P, Jiang H-W, Guo S-J, Le H-Y, Zhao X-D, Chen C-S, Zhang J-B, Tao S-C. (2012) Global identification of prokaryotic glycoproteins based on an Escherichia coli proteome microarray. PLoS ONE 7(11):e49080. (IF: 3.534)

10.      Guo S-L, Chen P-C, Chen M-S, Cheng Y-C, Lin J-M, Lee H-C, Chen C-S* (2012) A Fast Universal Immobilization of Immunoglobulin G at 4 °C for the Development of Array-based Immunoassays. PLoS ONE 7(12): e51370. (IF: 3.534) 指導學生為第五作者;指導博士後為共同第一作者

11.      Lu K-Y, Tao S-C, Yang T-C, Ho Y-H, Lee C-H, Lin C-C, Juan H-F, Huang H-C, Yang C-Y, Chen M-S, Lin Y-Y, Lu J-Y, Zhu H, Chen C-S* (2012) Profiling lipid-protein interactions using nonquenched fluorescent liposomal nanovesicles and proteome microarrays. Molecular & Cellular Proteomics 11(11):1177-90. (IF: 7.254) 指導學生為第一、第四作者

12.      Lin J-T, Chen P-C, Goud TV, Huang B-R, Lin T-C, Biellmann JF, Chen C-S* (2012) A Sulfhydryl-Reactive Ruthenium (II) Complex and its Conjugation to Protein G as a Universal Reagent for Fluorescent Immunoassays. PLoS ONE 7(4):e36086. (IF: 3.534) 指導學生為第一作者;指導博士後為共同第一作者

13.      Ho Y-H, Sung T-C, Chen C-S* (2012) Lactoferricin B inhibits the phosphorylation of two-component system response regulators: RasR and CreB. Molecular & Cellular Proteomics 11(4):M111.014720. (IF: 7.254) 指導學生為第一作者

14.      Horng H-C, Wen K-C, Su W-H, Chen C-S*, Wang P-H* (2012) Review of myomectomy. Taiwanese Journal of Obstetrics & Gynecology 51(1):7-11. (IF: 1.261) 指導學生為第三作者

15.      Tu Y-H, Ho Y-H, Chuang Y-C, Chen P-C, Chen C-S* (2011) Identification of Lactoferricin B Intracellular Targets Using an Escherichia coli Proteome Chip. PLoS ONE 6(12):e28197. (IF: 3.534) 指導學生為第一、第二作者

16.      Su W-H, Tsou T-S, Chen C-S, Ho T-Y, Lee W-L, Yu Y-Y, Chen T-H, Tan C-H, Wang P-H* (2011) Diagnosis of Chlamydia infection in women. Taiwanese Journal of Obstetrics & Gynecology 50(3):261-7. (IF: 1.261) 指導學生為第一、第四作者

17.      Su W-H, Tsou T-S, Chen C-S, Ho T-Y, Lee W-L, Yu Y-Y, Chen T-H, Tan C-H, Wang P-H* (2011) Are we satisfied with the tools for the diagnosis of gonococcal infection in females? Journal of the Chinese Medical Association 74(10):430-4. (IF: 0.889) 指導學生為第一、第四作者

18.      Sandy T, Chen C-S, Zhu H, Escalante-Semerena1 JC* (2010) Nε-Lysine Acetylation of a Bacterial Transcription Factor Inhibits Its DNA-Binding Activity. PLoS ONE 5(12): e15123 (IF: 3.534)

19.      Su W-H, Cheng M-H, Lee W-L, Tsou T-S, Chang W-H, Chen C-S* Wang P-H* (2010) Nonsteroidal anti-inflammatory drugs for wounds: pain relief or excessive scar formation? Mediators of Inflammation 2010: 1-8. Article ID 413238. (IF: 2.417) 指導學生為第一作者

20.      Chen M-S, Guo S-L, Cheng Y-C, Chen C-S* (2010) Comparison of Slide Surfaces for the Fabrication of Pain-Related Message Molecule Antibody Microarray. Chinese Journal of Pain 20:18-26.

21.      Chen C-S, Sullivan S, Anderson T, Tan AC, Alex PJ, Brant SR, Cuffari C, Bayless TM, Talor MV, Burek L, Wang H, Li R, Datta LW, Wu Y, Winslow RL*, Zhu H*, Li X* (2009) Identification of novel serological biomarkers for inflammatory bowel disease using E. coli proteome chip. Molecular & Cellular Proteomics 8:1765-1776. (IF: 7.254)

22.      Chen C-S, Korobkova E, Chen H, Zhu J, Jian X, Tao S-C, He C*, Zhu H* (2008) A proteome chip approach reveals new DNA base damage recognition activities in Escherichia coli. Nature Methods 5: 69-74. (IF: 25.953)

23.      Chen C-S, Baeumner AJ, Durst RA* (2008) Multiplexed immunoassays in food analysis. Handbook of Food Analysis Instruments Otles S (ed), CRC Press, Boca Raton, FL, p 439-454.

24.      Chen C-S, Tao S-C, Zhu H* (2008) Protein microarray technologies. Proteomics (Methods Express Series) O'Connor CD & Hames BD (eds), Scion Publishing Ltd., Bloxham, UK, p 183-205.

25.      Tao S-C, Chen C-S, Zhu H* (2007) Applications of protein microarray technology. Combinatorial Chemistry & High Throughput Screening 10(8): 706-718. (IF: 1.925)

26.      Chen C-S, Zhu H* (2006) Protein microarrays. Biotechniques 40:423-429. (IF: 2.754)

27.      Chen C-S, Durst RA* (2006) Simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes with an array-based immunosorbent assay using universal protein G-liposomal nanovesicles. Talanta 69: 232-238. (IF: 3.511)

28.      Chen C-S, Yao J, Durst RA* (2006) Liposome encapsulation of fluorescent nanoparticles: quantum dots and silica nanoparticles. Journal of Nanoparticle Research 8:1033-1038. (IF: 2.278)

29.      Chen C-S (2006) Liposomal nanovesicles for multiplexed immunoassays: universal protein G-liposomes and fluorescent nanoparticle-liposomes. Ph.D dissertation.

30.      Chen C-S, Baeumner AJ, Durst RA* (2005) Protein G-liposomal nanovesicles as universal reagents for immunoassays. Talanta 67: 205-211. (IF: 3.511)

31.      Chen C-S, Hsu C-K, Chiang B-H* (2002) Optimization of the enzymic process for manufacturing low-lactose milk containing oligosaccharides. Process Biochemistry 38: 801-808. (IF: 2.524)

32.      Chen R-H* and Chen C-S (2000) Changes of polydispersity and limiting molecular weight of ultrasonic treated chitosan. Advances in Chitin Science 4:361-366.

研究計畫區


Grant (PI)

1.          Fabrication of an Escherichia coli K1 proteome chip to study the interactions between human brain microvascular cell and Escherichia coli K1. NSC: 101-2320-B-008-004-MY3; 2012/08/01~2015/07/31

2.          利用人類蛋白質體晶片分析中風病人發病前血清自體抗體變化 NCU-LSH-101-A-024; 2012/01/01~2012/12/31 壢新合作計畫

3.          應用輪滾式擴增策略用於最佳化蛋白質 G 中介抗體微晶片平台 102NCU-CGH-07; 2013/01/01~2013/12/31 國泰合作計畫

4.          Deciphering the Viral RNA-host Interactions Using Human Proteome Microarrays. NHRI-EX102-10233SC; 2013/1/1~2016/12/31 國衛院計畫

5.          以高敏感C-反應蛋白和生物晶片找出可預測急性心肌梗塞發生之生物標記 NCU-LSH-102-A-019; 2013/01/01~2013/12/31 壢新合作計畫

6.          以蛋白質體陣列感測器系統化探討亞硫酸化後修飾之功能性蛋白質體─以蛋白質體微陣列晶片探討亞硫酸化蛋白質體學之作用機制 (子計畫二) (1/3) NSC 102-2627-M-008-001; 2013/08/01~2014/07/31

7.          以蛋白質體陣列感測器系統化探討亞硫酸化後修飾之功能性蛋白質體─以蛋白質體微陣列晶片探討亞硫酸化蛋白質體學之作用機制 (子計畫二) (2/3) MOST 103-2627-M-008-001; 2014/08/01~2015/07/31

8.          以蛋白質體陣列感測器系統化探討亞硫酸化後修飾之功能性蛋白質體─以蛋白質體微陣列晶片探討亞硫酸化蛋白質體學之作用機制 (子計畫二) (3/3) MOST 104-2627-M-008-001; 2014/08/01~2015/07/31

9.          Identification of intracellular target proteins of antimicrobial peptides using Escherichia coli K12 proteome chips. NSC: 97-2311-B-019-001-MY2; 2008/03/01~2009/10/31

10.      Systematic analysis of secretome network in human cerebrospinal fluid under distinct pain conditions using antibody microarrays. Cathay General Hospital; 2009/1/1~2009/12/31 國泰合作計畫

11.      Development and application of protein chips for a high-throughput systematic detection of interactions among proteins in cancer cells--Protein chips technology development. NSC 98-2627-M-008-004; 2009/08/01~2010/07/31

12.      Development and application of protein chips for a high-throughput systematic detection of interactions among proteins in cancer cells--Protein chips technology development. NSC 99-2627-M-008-003; 2010/08/01~2011/07/31

13.      Development and application of protein chips for a high-throughput systematic detection of interactions among proteins in cancer cells--Protein chips technology development. NSC 100-2627-M-008-003; 2011/08/01~2012/07/31

14.      Development of chip-based multiplexed immunoassays using liposomal nanovesicles for female Cervicitis. VGHUST99-G4-1; 2010/01/01~2010/12/31 榮台聯大計畫

15.      Investigation of the correlation between Rho-kinase activity and both diabetic clinic parameters as well as therapeutic drugs in diabetic patients. NCU-LSH-100-A-012; 2011/01/01~2012/03/31 壢新合作計畫

16.      Identification of Novel Serological Biomarkers for Bipolar Affective Disorder Using Proteome Chip. 100TMUH-NCU-005; 2011/06/01~2012/05/30 北醫合作計畫

17.      Construction of an Escherichia coli K1 proteome chip and its application on the novel assay development for the interaction study between human cells and pathogen proteome. NSC: 100-2320-B-008-001-; 2011/8/1~2012/7/31


Grant (co-PI)

1.          Deciphering the synergistic mechanisms of combined antimicrobial agents and discovering the new synergistic combinations using E. coli proteome microarrays. 104-2320-B-008-002-MY3; 2015/08/01~2018/07/31

2.          Systems biology approach and integrative platform for transcription and protein interaction network discovery: using novel oncoprotein ZNF322A as a model. (2/3) 103-2627-B-002-002-; 2014/08/01~2015/07/31

3.          Systems biology approach and integrative platform for transcription and protein interaction network discovery: using novel oncoprotein ZNF322A as a model. (1/3) 102-2627-B-002-002-; 2013/08/01~2014/07/31

4.          Potential role of host WWOX gene in the urinary tract infection by uropathogenic Escherichia coli. (2/3) 103-2320-B-006-003-; 2014/08/01~2015/07/31

5.          Potential role of host WWOX gene in the urinary tract infection by uropathogenic Escherichia coli. (1/3) 102-2320-B-006-020-; 2013/08/01~2014/07/31

6.          Application of proteomic microarray analysis of second trimester amniotic fluid antibodies in normal and abnormal pregnancy. 103-2314-B-182A-102-; 2014/08/01~2015/07/31

7.          Development of cancer pericellular fibronectin-targeted anti-metastatic protein drugs, cruise drug-delivery systems, and anti-metastatic cancer vaccines. (1/3) 103-2325-B-006-009-; 2014/05/01~2015/04/30

8.          Discovery and mechanism study of novel bacteriocins from lactic acid bacteria with the ability of inhibiting multidrug resistant pathogens. 102-2313-B-424-003-MY2; 2013/10/01~2015/07/31

9.          Targeting Cancer Pericellular Fibronectin with Dipeptidyl Peptidase IV-Derived Peptides: Detection, Prevention, and Treatment of Cancer Metastasis. 102-2320-B-006-031-; 2013/08/01~2014/07/31

10.      Monoamine Oxidase Inhibitors as Novel Treatments for Prostate Cancer. 101-2314-B-010-071-MY3; 2012/10/01~2015/09/30

11.      以大腸癌發展擷取、辨識和免疫分型癌症幹細胞103CGH-NCU-A3; 01/01/2014~12/31/2014 國泰合作計畫

12.      使用基因組外遺傳小分子重新編程化學誘導型多能性幹細胞 NCU-LSH-103-A-001; 01/01/2014~12/31/2014 壢新合作計畫

專利區

 Patent

ASSAY KIT AND ANALYSIS METHOD (分析套組及分析方法), Taiwan I472369 (20150211-20320723) and US 20140030745 A1 (in application).